Crystallization of cowpea chlorotic mottle virus and belladonna mottle virus

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The structure of cucumber mosaic virus and comparison to cowpea chlorotic mottle virus.

The structure of cucumber mosaic virus (CMV; strain Fny) has been determined to a 3.2-A resolution using X-ray crystallography. Despite the fact that CMV has only 19% capsid protein sequence identity (34% similarity) to cowpea chlorotic mottle virus (CCMV), the core structures of these two members of the Bromoviridae family are highly homologous. As suggested by a previous low-resolution struct...

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Replication and Properties of Cowpea Chlorotic Mottle Virus in Resistant Cowpeas

WYATT, S. D., and C. W. KUHN. 1979. Replication and properties of cowpea chlorotic mottle virus in resistant cowpeas. Phytopathology 69:125129. Although cowpea chlorotic mottle virus (CCMV) caused no symptoms pool of RNA formed soon after inoculation. Several properties of CCMV on cowpea P1 186465, virus could be isolated from the inoculated primary produced in the resistant plants were similar...

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Structural rigidity in the capsid assembly of cowpea chlorotic mottle virus

The cowpea chlorotic mottle virus (CCMV) has a protein cage, or capsid, which encloses its genetic material. The structure of the capsid consists of 180 copies of a single protein that self-assemble inside a cell to form a complete capsid with icosahedral symmetry. The icosahedral surface can be naturally divided into pentagonal and hexagonal faces, and the formation of either of these faces ha...

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Dispensability of 3' tRNA-like sequence for packaging cowpea chlorotic mottle virus genomic RNAs.

The 3' ends of three genomic RNAs (gRNAs) of cowpea chlorotic mottle virus (CCMV) terminate in a highly conserved tRNA-like structure (3'TLS). To examine the intrinsic role played the 3'TLS in packaging, the competence of each gRNA lacking the 3' TLS (DeltaTLS-gRNA) to interact with dissociated coat protein (CP) subunits and form virions was assayed in vitro. In contrast to the well established...

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Feasibility of Cowpea chlorotic mottle virus-like particles as scaffold for epitope presentations

BACKGROUND & METHODS Within the last decade Virus-Like Particles (VLPs) have increasingly received attention from scientists for their use as a carrier of (peptide) molecules or as scaffold to present epitopes for use in subunit vaccines. To test the feasibility of Cowpea chlorotic mottle virus (CCMV) particles as a scaffold for epitope presentation and identify sites for epitope fusion or inse...

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ژورنال

عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography

سال: 1981

ISSN: 0108-7673

DOI: 10.1107/s0108767381098152